PTEN gene and phosphorylation of Akt protein expression in the LPS-induced lung fibroblast

Mao-lin Huang, Zhen-dong Yang, Lu-lu Gong, Ning Cui

Abstract


Objective: To investigate PTEN gene expression and the Akt phosphorylation of protein expression in the LPS-induced lung fibroblast, to initially reveal the relation between PTEN gene and the Akt phosphorylated proteins to LPS-induced lung fibroblast proliferation mechanism. Methods: BrdU experiments was performed to evaluate the LPS-induced lung fibroblast proliferation,  RT-PCR and Western Blot analysis were used to analyze the PTEN gene expression and Western blot was performed to analyze Akt phosphorylated protein expression. Results: PTEN mRNA level of the experimental group were significantly lower than the control group (P<0.05) with LPS simulation for 24h and 72h , and there were no significant difference between the experimental group and control group the experimental group and control group (P>0.05) . PTEN protein expression levels of the experimental group were significantly lower than the control group (P<0.05) , at 72h, and PTEN mRNA levels had no significant differences between these of the experimental and control group at 6h,12h and 24h(p>0.05). Phosphorylation Akt protein level (relative to total Akt protein) was significantly higer than the control group (P<0.05) at 24h and 72h, and phosphorylation Akt protein levels had no significant differences between these of the experimental and control group at 6h and 12h (P>0.05) .Conclusion: PTEN gene and phosphorylation Akt protein involve in LPS-induced lung fibroblast proliferation signal transduction pathway.


Keywords


Pulmonary fibrosis cells; Lipopolysaccharide; Cell proliferation; Protein kinase B

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DOI: http://dx.doi.org/10.14725/gjicmwm.v2n3a585

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Global Journal of Integrated Chinese Medicine and Western Medicine

ISSN 2308-6025 (Print)  ISSN 2375-4710 (Online)