The function of microparticles and the research progress of diabetes and microparticles

Min-dan Xu, Ke-qin Zhang


MPs are vesicles released by cells when stimulated by physical (e.g. shear force) or chemical (e.g. agonists) factors, as well as cells undergoing apoptosis or exposed to inflammatory conditions . MPs are 100~1000 nm in diameter, have membrane cytoskeletons, express phosphatidylserine (PS) on the surface, and lack of nuclei. Surface molecules, enzymes, RNA and DNA are conveyed via MPs from origin cells to target cells. As mediators of information transfer, MPs have been proposed to impose pro-inflammatory and pro-coagulant effects in many disease states, such as cancer, venous thromboembolism, arteriosclerosis, and diabetes mellitus. The hypercoagulable state associated with diabetes is well recognized. More T2DM patients have died from thrombotic diseases. The endothelium-derived MPs in diabetic patients were elevated. TF-positive MPs concentration was increased and procoagulant activity of MPs was elevated. It is worth to research the role of MPs in the hypercoagulable state of diabetic patients.


Microparticles; Tissue factor; Diabetes; Hypercoagulable

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Hugel B, Martinez C, Kunzelmann C, et al. Membrane microparticles: two sides of the coin[J]. Physiology, 2005, 20:22-27.

Enjeti AK, Lincz LF, Seldon M. Detection and measurement of microparticles: An evolving research tool for vascular biology[J]. Semin Thromb Hemost , 2007, 33(8) : 771-779.

Wolf P. The nature and significance of platelet products in human plasma[J]. Br J Haematol, 1967, 13: 269-288.

Orozco A F, Lewis D E. Flow cytometric analysis of circulating microparticles in plasma[J]. Cytometry A, 2010, 77:502-514.

Banfi C, Brioschi M, Wait R, et al. Proteome of endothelial cell-derived procoagulant microparticles[J]. Proteomics, 2005, 5(17): 4443-4455.

Dignat-George F, Boulanger C M. The many faces of endothelial microparticles[J]. Arterioscler Thromb Vasc Biol, 2011, 31, 27-33.

Morel O, Jesel L, Freyssinet JM, et al. Cellular mechanisms underlying the formation of circulating microparticles[J]. Arterioscler Thromb Vasc Biol, 2011, 31:15-16.

Berckmans RJ, Nieuwlands R, Boing AN, et al. Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation[J]. Thromb Haemost, 2001, 85: 639-646. PMid:11341498

Rautou PE, Vion AC, Amabile N, et al. Microparticles, vascular function, and atherothrombosis[J]. Circ Res, 2011, 109: 593-606.

Muralidharan-Chari V, Clancy JW, Sedgwick A, et al. Microvesicles: mediators of extracellular communication during cancer progression[J]. J Cell Sci, 2010, 123: 1603-1611.

Cauwenberghs S, Feijge MA, Harper AG, et al. Shedding of procoagulant microparticles from unstimulated platelets by integrin-mediated destabilization of actin cytoskeleton[J]. FEBS Lett, 2006, 580: 5313-5320.

Owens AP, 3rd,Mackman N. Microparticles in hemostasis and thrombosis[J]. Circ Res, 2011, 108: 1284-1297.

Key NS. Analysis of tissue factor positive microparticles[J]. Thromb Res, 2010, 125:s42-s45.

Morel O, Toti F, Hugel B, et al. Procoagulant microparticles: Disrupting the vascular homeostasis equation[J]? Arterioscler Thromb Vasc Biol, 2006, 6:2594-2604.

Wiiger MT, Prydz H. The changing faces of tissue factor biology. A personal tribute to the understanding of the “extrinsic coagulation activation” [J]. Thromb Haemost, 2007, 98:38-42.

Mackman N, Tilley RE, Key NS. Role of the extrinsic pathway of blood coagulation in hemostasis and thrombosis[J]. Arterioscler Thromb Vasc Biol, 2007, 27:1687-1693.

Giesen PL, Rauch U, Bohrmann B, et al. Blood-borne tissue factor: another view of thrombosis[J]. Proc Natl Acad Sci U S A, 1999, 96:2311-2315.

Chou J, Mackman N, Merrill-Skoloff G, et al. Hematopoietic cell-derived microparticle tissue factor contributes to fibrin formation during thrombus propagation[J]. Blood, 2004, 104:3190-3197.

Siddiqui FA, Desai H, Amirkhosravi A, et al. The presence and release of tissue factor from human platelets[J]. Platelets, 2002, 13: 247-253.

Garcia BA, Smalley DM. The platelet microparticle proteome[J]. J Proteome Res, 2005, 4: 1516-1521.

Gilbert GE, Sims PJ, Wiedmer T, et al. Platelet-derived microparticles express high affinity receptors for factor Ⅷ[J]. J Biol Chem, 1991, 266: 17261-17268.

Comfurius P, Senden JMG, Tilly RHJ, et al. Loss of membrane phospholipid asymmetry in platelets and red cells may be associated with calcium-induced shedding of plasma membrane and inhibition of aminophospholipid translocase[J]. Biopchim Biophys Acta, 1990, 1026:153 -160.

Hoffnan M, Monroe DM, Roberts HR. Coagulation factor Ⅸa binding to activated platelets and platelet-derived microparticles: a flow cytometric study[J]. Thromb Haemost, 1992, 68: 74-78.

Sinauridze EI, Kireev DA, Popenko NY, et al. Platelet microparticle membranes have 50- to 100-fold higher specific procoagulant activity than activated platelets[J]. Thromb Haemost, 2007, 97: 425-434.

Johnson GJ, Leis LA, Bach RR. Tissue factor activity of blood mononuclear cells is increased after total knee arthroplasty[J]. Thromb Haemost, 2009, 102:728-734.

Losche W. Platelets and tissue factor[J]. Platelets, 2005, 16: 313-319.

Del Conde I, Shrimpton CN, Thiagarajan P, et al. Tissue-factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation[J]. Blood, 2005, 106: 1604-1611.

Nielsen CT, Ostergaard O, Johnsen C, et al. Distinct features of circulating microparticles and their relationship to clinical manifestations in systemic lupus erythematosus[J]. Arthritis Rheum, 2011, 63(10): 3067-3077.

Abatier F,Darmon P,Hugel B,et al. Type l and type 2 diabetic patients display different patterns of cellular microparticles[J]. Diabetes, 2002, 51(9): 2840-2845.

Jimenez JJ,Jy W,Mauro LM,et al.Elevated endothelial microparticles in thrombotic thrombocytopenic purpura:findings from brain and renal microvascular cell culture and patients with active disease[J].Br J Haematol, 2001, 112(1): 81-90.

Kagawa H, Komiyama Y, Nakamura S, et al. Expression of functional tissue factor on small vesicles of lipopolysaccharide-stimulated human vascular endothelial cells[J]. Thromb Res, 1998, 91: 297 - 304.

Nomura S, Shouzu A, Omoto S, et al. Activated platelets and oxidized LDL induce endothelial membrane vesiculation: clinical significance of endothelial cell-derived microparticles in patients with type 2 diabetes[J]. Clin Appl Thromb/Hemost, 2004, 10: 205 - 215.

Jy W, Jimenez JJ, Mauro LM, et al. Endothelial microparticles induce formation of platelet aggregates via a von Willebrand factor/ristocetin dependent pathway, rendering them resistant to dissociation[J]. Thromb Haemost, 2005, 3: 1301-1308.

Nomura S, Ozaki Y, Ikeda Y. Function and role of microparticles in various clinical settings[J]. Thromb Res, 2008, 123: 8-23.

Horstman LL, Jy W, Jimenez JJ, et al. Endothelial microparticles as markers of endothelial dysfunction[J]. Front Biosci, 2004, 9: 118-135.

Lacroix R, Dignat-George F. Microparticles as a circulating source of procoagulant and fibrinolytic activities in the circulation[J]. Thromb Res, 2012, 129: s27-29.

Kushak RI, Nestoridi E, Lambert J, et al. Detached endothelial cells and microparticles as sources of tissue factor activity[J]. Thromb Res, 2005, 116: 409-419.

Aharon A, Katzenell S, Tamari T, et al. Microparticles bearing tissue factor and tissue factor pathway inhibitor in gestational vascular complications[J]. J Thromb Haemost, 2009, 7: 1047-1050.

Steppich B, Mattisek C, Sobczyk D, et al. Tissue factor pathway inhibitor on circulating microparticles in acute myocardial infarction[J]. Thromb Haemost, 2005, 93: 35-39.

Tsimerman G, Roguin A, Bachar A, et al. Involvement of microparticles in diabetic vascular complications[J]. Thromb Haemost, 2011, 106: 310-321.

Aharon A, Brenner B. Microparticles, thrombosis and cancer[J]. Best Pract Res Clin Haematol, 2009, 22: 61-69.

Perez-Casal M, Downey C, Fukudome K, et al. Activated protein C induces the release of microparticle-associated endothelial protein C receptor[J]. Blood, 2005, 105: 1515-1522.

Van Beers EJ, Schaap MC, Berckmans RJ, et al. Circulating erythrocyte-derived microparticles are associated with coagulation activation in sickle cell disease[J]. Haematologica, 2009, 94: 1513-1519.

Shah MD, Bergeron AL, Dong JF, et al. Flow cytometric measurement of microparticles: pitfalls and protocol modifications[J]. Platelets, 2008, 19: 365-372.

Connor DE, Exner T, Ma DD, et al. Detection of the procoagulant activity of microparticle-associated phosphatidylserine using XACT[J]. Blood Coagul Fibrinolysis, 2009, 20: 558-564.

Rubin O, Crettaz D, Tissot JD, et al. Pre-analytical and methodological challenges in red blood cell microparticle proteomics[J]. Talanta, 2010, 82: 1-8.

Lacroix R, Judicone C, Mooberry M, et al. The, Isth S. S. C. Workshop. Standardization of pre-analytical variables in plasma microparticle determination: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop[J]. J Thromb Haemost, 2013, 11: 1190-1193.

Haffner SM, Lehto S, Ronnemaa T, et al. Mortality from coronary heart disease in subjects with type 2 diabetes and in nondiabetic subjects with and without prior myocardial infarction[J]. N Engl J Med, 1998, 339: 229-234.

Vazzana N, Ranalli P, Cuccurullo C, et al. Diabetes mellitus and thrombosis[J]. Thromb Res, 2012, 129(3): 371-377.

Hamed S, Brenner B, Roguin A. Nitric oxide: a key factor behind the dysfunctionality of endothelial progenitor cells in diabetes mellitus type-2[J]. Cardiovasc Res, 2011, 91: 9-15.

Alzahrani SH, Ajjan RA. Coagulation and fibrinolysis in diabetes[J]. Diab Vasc Dis Res, 2012, 7(4): 260-273.

Sabatier F, Darmon P, Hugel B, et al. Type 1 and type 2 diabetic patients display different patterns of cellular microparticles[J]. Diabetes, 2002, 51(9): 2840-2845.

Tramontano AF, Lyubarova R, Tsiakos J, et al. Circulating Endothelial Microparticles in Diabetes Mellitus[J]. Mediators Inflamm, 2010, 16: 250476.

Zhang X, McGeoch SC, Johnstone AM, et al. Platelet-derived microparticle count and surface molecule expression differ between subjects with and without type 2 diabetes, independently of obesity status[J]. J Thromb Thrombolysis, 2014,37(4): 455-463.

Salem MA, Adly AA, Ismail EA, et al. Platelets microparticles as a link between micro- and macro-angiopathy in young patients with type 1 diabetes[J]. Platelets, 2015, 25: 1-7.

Tsimerman G, Roguin A, Bachar A, et al. Involvement of microparticles in diabetic vascular complications[J]. Thromb Haemost, 2011, 106(2): 310-321.

Diamant M. Elevated Numbers of Tissue-Factor Exposing Microparticles Correlate With Components of the Metabolic Syndrome in Uncomplicated Type 2 Diabetes Mellitus[J]. Circulation, 2002, 106(19): 2442-2447.

Cheng F, Wang Y, Li J, et al. Berberine improves endothelial function by reducing endothelial microparticles-mediated oxidative stress in humans[J]. Int J Cardiol, 2013, 169(3):936-942.



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Global Journal of Endocrinology and Metabolism Studies

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